Report no. 402


05.10.1998
Bact-a-cid for finishers

Abstract
The products Bact-a-cid and a modified version of the same product were tested for finishers from 27 to 107 kg live weight in one herd.

The products were compared to a control feed without antibiotic growth promoter. This trial was carried out in cooperation with Agil and Nurtiscan A/S, who supplied the products tested. In the trial there were 12 repetitions of each treatment and in total 120 pigs per group.

The trial included the following treatments:
- Control feed without growth promoter
- Bact-a-cid (dosage 3 kg per tonne for growers and 2.5 kg per tonne for finishers)
- Modified Bact-a-cid (dosage 3 kg per tonne for growers and 2.5 kg per tonne for finishers).

The result of the chemical analysis of the feed showed a good concordance with the calculated nutritional content.

The production value of the individual diets was calculated on the basis of the production results achieved and stated as gross margin per pen per year at equal prices for the feed.

The trial showed that addition of Bact-a-cid and Modified Bact-a-cid to finisher feed did not result in any significant effect on the production value when compared to the control group without growth promoter. Neither was there a difference between the two tested products.

Background
The use of antibiotic growth promoter in feed is presumed to increase the risk of developing bacteria, resistant to antibiotics used in human medicine. Therefore, it is a desire to find alternative products which can improve feed conversion and daily gain.

The market offers an increasing number of products as alternative to growth promoters. Documentation for the effectiveness of the products is, however, necessary to be able to estimate the financial gain of using the products in feed for finishers. The object of testing commercial products for finishers is to provide documentation for the production value of the products. The effect of the products is measured by the production results: feed conversion, daily gain and lean meat percentage.

The purpose of the trial was to examine if the products Bact-a-cid and Modified Bact-a-cid with a dosage of 3 and 2.5 kg per tonne of growers and finisher feed, respectively, resulted in an improved production value compared to a control feed without growth promoter.

The effect of the product was measured as the gross margin per pen per year, which were calculated on the basis of the production results: feed conversion ratio, daily weight gain and lean meat percentage.

Materials and methods
This trial was carried out in one herd with finishers, where addition of Bact-a-cid and Modified Bact-a-cid were compared to a control feed without growth promoter. Moreover the two products were compared mutual. The experimental design is shown in table 1.



Table 1. Experimental design

Group

1

2

3

Grower feed (27-57 kg)

Control

0.30% Bact-a-cid

0.30% Modified
Bact-a-cid

Finisher feed (57-107 kg)

Control

0.25% Bact-a-cid

0.25% Modified
Bact-a-cid



All feed mixtures were produced at Aarhusegnens Andels Grovvareforening a.m.b.a. A description of the products tested is shown in appendix 3. In all groups the feed was heat treated and pelleted at a minimum temperature of 81 degrees Celsius. Grower and finisher feed were produced in one and three batches, respectively. At each delivery all three mixes were tested for content of energy (EFOS), amino acids, calcium, phosphor and copper. The starter diet was screened for antibiotics. The content of essential amino acids in both grower and finisher diet were 5 per cent above the recommendations. This was done in order to counteract fluctuation in the crude protein contents of the raw materials.

The trial was carried out in a herd which had conventional health status and own production of piglets. The finisher herd had fully slatted floor. The pigs entered the trial at an average live weight of 27 kg. In total 360 pigs in the trial were distributed in 12 blocks (repetitions), in total 120 pigs per treatment. When entering the trial the pigs were divided according to sex firstly and then after weight in groups of 30 pigs. The 30 biggest castrates were distributed randomly in block 1 and the next biggest castrates were distributed randomly in block 2 and so on. There were 10 pigs in each pen. Within each block (three pens) the distribution of sexes were equal.

Each pen had one simple dry feed dispenser and one nipple drinker. The pigs were fed ad libitum and had access to feed day and night. Feed was dispensed manually twice a day. The pigs were weighted when shifting the feed approximately 5 weeks after the start of the trial.

The primary parameters in the analysis of production value were daily weight gain, feed conversion rate, carcass weight and lean meat percentage. As secondary parameters - registration of treatment of diseases as well as dead were analysed. Data were subjected to an analysis of variance according to GLM procedure in SAS. The production value stated as gross margin per pen per year was calculated based on the production results achieved and was adjusted to the same weight at entry and at slaughter.

The gross margin per pen per year was calculated as:


Gross margin per pig

=

Sales price ÷ purchase price ÷ feed expenses ÷ various expenses.

Gross margin per pen per year

=

Gross margin per pig x (365 days/feeding days per pig) x per cent utilization of herd.



The following assumptions have been used in the calculations: average price for piglets the last 12 months: 342 DKK per 30 kg piglet (±4,82 DKK per kg 25-30 kg) and for finishers 9.65 DKK per kg slaughter weight. The feed prices used were 1.35 and 1.23 DKK per FUs for growers and finishers, respectively, and were calculated as an average of the prices the last 12 months. Various expenses amount to 20 DKK per pig and the utilization of the pens was calculated as 95 per cent.

The following class variables were included in the statistical model: block and group. Three comparisons in pair were made (group 1 against 2, group 1 against 3 and group 2 against 3). Statistically significant differences are stated at 5 per cent level and are corrected for the three comparison in pairs by a Bonferroni t-test.

Results and discussion
Feed analysis
The composition of the feed mixtures is shown in appendix 1 and the chemical analysis of the feed is shown in appendix 2. The results of the chemical analysis of the grower and finishers feed showed good concordance with the calculated nutritional content.

Treatment of disease
No differences between the groups in frequency of treatments or the number of dead and culled pigs within the three groups were found.

In the period from insertion until intermediate weighing, three pigs died in group 3. In the period from intermediate weighing until slaughter two pigs died in group 3. In the control group, group 2 and 3, four, six and four pigs, respectively, were culled from the trial.

Production results
The production results are shown for the period before and after the intermediate weighting as well as for the whole test period (table 2). The results are stated as average corrected to the same weight at insertion, intermediate weighing and at slaughter.


Table 2. Production results corrected to the same weight at entry, at intermediate weighting and at delivery.

Feed mixture

1
Control without growth promoter

2
Bact-a-cid

3
Modified
Bact-a-cid

Number of blocks (replications)
Number of pigs delivered

12
116

12
114

12
112

27-57 kg:
Daily feed intake, FUs
Daily weight gain, g
FUs per kg weight gain


1.65
674
2.44


1.58
687
2.29


1.69
706
2.39

57-107 kg:
Daily feed intake. FUs
Daily weight gain. g
FUs per kg weight gain


2.78
990
2.81


2.78
971
2.86


2.80
964
2.90

27-107 kg:
Daily feed intake. FUs
Daily weight gain. g
FUs per kg weight gain
Lean meat percentage


2.25
840
2.67
59.6


2.22
839
2.65
59.6


2.29
846
2.71
59.3



The production values stated as gross margin per pen place per year are shown in table 3. The production values were calculated on the basis of the production results achieved (daily weight gain, FUs per kg weight gain and lean meat content). The price for addition of the products is not included.

The actual production value where the sales price of the products tested has been added to the price of the control feed without growth promoter is stated in table 3. The price for the two products tested was stated by the supplier of the product and reflects the price which the pig producer has to pay for the products, inclusive profit for the feedstuff companies.



Table 3. Production value and gross margin at actual prices

Feed mixture

1
Control without
growth promoter

2
Bact-a-cid

3
Modified
Bact-a-cid

Production value:
Gross margin/pen place/year 1),
DKK 2)
Index 2)



634a
100



646a
102



615a
97

Actual production value
gross margin/pen place/year 3)
Index


634
100


620
98


588
93





1)

Daily weight gain and feed conversion rate is adjusted to the same weight at entry and at slaughter

2)

There has to be a difference in gross margin per pen place per year at minimum 53 DKK or 8 index points, before there is a significant difference between the groups.

3)

There is not performed a statistical calculation on the differences in the gross margin.



In the growth period from 27 to 57 kg, group 2 had a lower feed intake and feed conversion ratio, than the other groups. This difference was not maintained in the last growth period from 57 to 107 kg. In total addition of the product Bact-a-cid and Modified Bact-a-cid for growing and finishing pigs did not show any significant difference in the production value. Neither was there a significant difference in production value when comparing the two products.

Appendix 1
Grower, mixes ingredients percentage

Group

1
Control

2
Bact-a-cid

3
Modified
Bact-a-cid

Wheat

45.00

45.02

45.02

Soya meal toasted

26.43

26.54

26.54

Barley

22.00

21.48

21.48

Animal fat

2.25

2.36

2.36

Molasses

1.50

1.50

1.50

Chalk

1.19

1.18

1.18

Mocalciumphosphate

0.71

0.71

0.71

NaCl

0.40

0.40

0.40

LKF special finisher

0.20

0.20

0.20

L-Lysine hydrochloride

0.13

0.13

0.13

DL-Methionine 40 %

0.13

0.12

0.12

L-Threonine 50 %

0.06

0.06

0.06

Bact-a-cid

-

0.30

-

Modified Bact-a-cid

-

-

0.30



Finishers, mixes ingredients percentage

Group

1
Control

2
Bact-a-cid

3
Modified
Bact-a-cid

Wheat

26.00

26.00

26.00

Soya meal toasted

19.51

19.47

19.47

Barley

48.81

48.6

48.60

Animal fat

1.50

1.50

1.50

Molasses sugar canes

1.50

1.50

1.50

Chalk

1.22

1.22

1.22

Monocalciumphosphat

0.62

0.62

0.62

NaCl

0.43

0.43

0.43

LKF-special finisher

0.20

0.20

0.20

L-Lysine hydrochloride

0.12

0.12

0.12

DL-Methionine 40 %

0.07

0.07

0.07

L-Threonine 50 %

0.02

0.02

0.02

Bact-a-cid

-

0.25

-

Modified Bact-a-cid

-

-

0.25



Appendix 2
Calculated and analysed nutritional content of the mixes for growers - one delivery

Group
Mix

All groups

1
Control

2
Bact-a-cid

3
Modified
Bact-a-cid

Calculated / analysed 1)

Calculated

Analysed

Analysed

Analysed

FUs per 100 kg 2)

110

110

111

110

Crude protein, % 2)

19.1

19.2

19.4

19.4

Lysine, g/kg

10.4

10.7

10.7

10.5

Methionine, g/kg

3.3

3.3

3.5

3.4

Threonine, g/kg

7.0

7.1

7.3

7.3

Calcium, g/kg

7.7

7.0

7.1

7.1

Total-phosphor, g/kg

5.7

5.7

5.7

5.5

Copper, mg/kg

31

39.0

35.0

29.0

Antibiotic screening

-

negative

negative

negative


1) 

Analysed content is based on one analysis if nothing else is mentioned.

2)

 Average of four analyses.



Calculated and analysed nutritional content of the mixes for finishers - three deliveries

Group:
Mix:

All groups

1
Control

2
Bact-a-cid

3
Modified
Bact-a-cid

Calculated / analysed 1)

Calculated

Analysed

Analysed

Analysed

FUs per 100 kg 2)

105

108

108

108

Crude protein, % 2)

16.7

17.0

17.1

17.0

Lysine, g/kg

8.9

9.2

8.9

8.9

Methionine, g/kg

2.8

2.7

2.7

2.6

Threonine, g/kg

6.0

6.3

6.0

6.0

Calcium, g/kg

7.4

7.8

6.9

7.5

Total-phosphor, g/kg

5.4

5.9

5.1

5.4

Cobber, mg/kg

29

35

33

27


1) 

Analysed content is an average of three analyses if nothing else is mentioned.

2)

Average of six analyses.



Appendix 3 - Product description based on company information´

Products:

Bact-a-cid and Modified Bact-a-cid

Supplier: 




Agil
c/o Nutriscan A/S
Rørsangervej 8
8300 Odder
Denmark
Tel: +45 86 54 24 88

Bact-a-cid
Contents:
 

A combination of the following acids: Formic acid; ammoniunformiate, propione acid, ammoniumpropionate. Dosage 0.2-0.4 %.

Price:

13 DKK per kg product corresponding to: 3.90 and 3.25 DKK per 100 kg feed when the dosage is 0.30% and 0.25% for growers and finishers, respectively.

Modified Bact-a-cid
Contents:

A combination of the following protected acids: Formic acid, propione acid and metylpropione acid. Dosage 0.2-0.4%.

Price:

13.50 DKK per kg of the product corresponding to: 4.05 and 3.38 DKK per 100 kg feed when the dosage is 0.30% and 0.25% for growers and finishers, respectively.





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